QX200™ ddPCR™ EvaGreen Supermix from Bio-Rad

Product Specs
ItemQX200™ ddPCR™ EvaGreen Supermix
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1864033
Quantity200 rxns
ApplicationsPCR
Sample TypecDNA, genomic DNA, plasmid DNA
TypeSupermix
Brand/TrademarkLSG DBG

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Extracellular vesicle-derived CircWhsc1 promotes cardiomyocyte proliferation and heart repair by activating TRIM59/STAT3/Cyclin B2 pathwayJournal of advanced researchNovember 1, 2023Wei G, Li C, Jia X, Xie J, Tang Z, Jin M, Chen Q, Sun Y, He S, Li X, et al.digital PCR (ddPCR) Droplet digital PCR was performed using 10 μL QX200 EvaGreen ddPCR Supermix (#1864033, Bio-Rad), 2 μL cDNA, primer set (final concentration: 100 nM), and ddH2O, making a 20 μL reaction.

(2) Yeast transcription factor Msn2 binds to G4 DNANucleic Acids ResearchOctober 13, 2023Duong Long Duy, Nayun Kimby Bio-rad QX200 Droplet Digital PCR (ddPCR) system and QX200 ddPCR EvaGreen Supermix (Bio-Rad, Cat# 1864035). The ddPCR primers are listed in Supplementary Table S2. The mRNA fold change was calculated by normalizing

(3) Antimicrobial and anticarcinogenic activity of bioactive peptides derived from abalone viscera (Haliotis fulgens and Haliotis corrugata)Scientific ReportsSeptember 13, 2023Crisalejandra Rivera-Pérez, Xolotl Paloma Ponce González, Norma Yolanda Hernández-Savedraeach 20 µL reactions consisted of 1X EvaGreen ddPCR Supermix (QX200 ddPCR EvaGreen Supermix, Cat. No. 1864033, Bio-Rad), 100 nM gene-specific primers (Table 2), and 1 µL of the cDNA sample (100 ng). The reaction

(4) Antibiotic resistance gene sequencing is necessary to reveal the complex dynamics of immigration from sewers to activated sludgeFrontiers in MicrobiologyApril 26, 2023Claire Gibson, Susanne A. Kraemer, Natalia Klimova, Bing Guo, Dominic Frigondigital PCR reaction, which included 1 × QX200™ ddPCR™ Evagreen® Supermix (Bio-Rad, CA, USA; catalogue number 1864033), 100 nM of the forward and reverse primers, template DNA as determined using equation 1, and RNase/DNase-free

(5) Improper preanalytical processes on peripheral blood compromise RNA quality and skew the transcriptional readouts of mRNA and LncRNAFrontiers in GeneticsJanuary 4, 2023Yinli He, Lele Dong, Hongyang Yi, Linpei Zhang, Xue Shi, Lin Su, Baoyu Gan, Ruirui Guo, Yawen Wang, Qinying Luo, et al.Cat: FSQ-101). The aqueous ddPCR mixture containing 10 µl of ddPCR™ EvaGreen Supermix (BIO-RAD, Cat: #1864033), 3 µl of β-actin primers (3.75 µM) and 7 µl of cDNA was emulsified into picoliter droplets of thermostable

(6) Overexpression of Wild‐Type ACVR1 in Fibrodysplasia Ossificans Progressiva Mice Rescues Perinatal Lethality and Inhibits Heterotopic OssificationJournal of Bone and Mineral ResearchNovember 1, 2022Masakazu Yamamoto, Sean J Stoessel, Shoko Yamamoto, David J Goldhamertranscripts was determined by Bio‐Rad QX200 Droplet Digital PCR system using QX200 ddPCR EvaGreen Supermix (#1864033) according to the manufacturer's instructions. The following PCR primer sets were used for each gene

(7) A bidirectional competitive interaction between circHomer1 and Homer1b within the orbitofrontal cortex regulates reversal learningCell ReportsJanuary 18, 2022Alexander K. Hafez et al.DG8™ Cartridges for QX200™/QX100™ Droplet Generator, X200™Droplet Generation Oil for EvaGreen",Bio-Rad Laboratories | Life Science Group,"Cat#: 1864033, 1864008, 1864005" M-MLV Reverse Transcriptase (200 U/μL),Invitrogen™,Cat#: 28025013 ,, Deposited

(8) Viral tropism and detection of clade 2.3.4.4b H5N8 highly pathogenic avian influenza viruses in feathers of ducks and geeseScientific ReportsDecember 1, 2021Nicolas Gaide et al.samples and controls were used for PCR amplification with the QX200 ddPCR EvaGreen Supermix (Bio-Rad 1864033) and M and H5 primers previously described. ddPCR were processed according to the instruction for QX200 AutoDG Droplet Digital PCR System

(9) The Fkh1 Forkhead associated domain promotes ORC binding to a subset of DNA replication origins in budding yeastNucleic Acids ResearchOctober 11, 2021Timothy Hoggard, Allison J Hollatz, Rachel E Cherney, Melissa R Seman, Catherine A FoxDNA replication (30) using Evagreen based chemistry (Droplet oil and Supermix; BioRad #1864005 and #1864033, respectively). ddPCR was performed on the indicated origins or non-origin control loci. Primers are

(10) Variation in human herpesvirus 6B telomeric integration, excision, and transmission between tissues and individualseLifeSeptember 21, 2021Michael L Wood et al.mix,Bio-Rad Laboratories,10031243, Commercial assay or kit,ddPCR EvaGreen Supermix,Bio-Rad Laboratories,1864033, Commercial assay or kit,Zymoclean gel DNA recovery kit,"Zymo Research, Irvine, CA",D4002, "Software,

(11) Predominantly inverse modulation of gene expression in genomically unbalanced disomic haploid maizeThe Plant CellMay 31, 2021Hua Yang, Xiaowen Shi, Chen Chen, Jie Hou, Tieming Ji, Jianlin Cheng, James A Birchlergenome can be determined. ddPCR The ddPCR reaction mixture was prepared from a 2× ddPCR EvaGreen mix (1864033, Bio-Rad), primers (250 nM primers) and template (50 ng cDNA+gDNA mix) in a final volume of 22 μL; 20

(12) hnRNPK-regulated LINC00263 promotes malignant phenotypes through miR-147a/CAPN2Cell Death & DiseaseApril 1, 2021Woo Joo Lee, Chang Hoon Shin, Haein Ji, Seong Dong Jeong, Mi-So Park, Hong-Hee Won, Poonam R. Pandey, Dimitrios Tsitsipatis, Myriam Gorospe, Hyeon Ho KimFirst-Strand Synthesis kit. The PCR reaction using the droplets was generated using EvaGreen Supermix (1864033, Bio-Rad) containing 1 μl of cDNA for quantifying the copy numbers of LINC00263 and miR-147a. In both

(13) Autophagy is associated with a robust specific transcriptional signature in breast cancer subtypesGenes & CancerDecember 31, 2020Céline Grandvallet, Jean Paul Feugeas, Franck Monnien, Gilles Despouy, Perez Valérie, Guittaut Michaël, Eric Hervouet, Paul Peixotousing the QX200 Droplet Digital PCR system (ddPCR, Biorad) with the QX200 ddPCR EvaGreen Supermix (1864033, Biorad) according to the manufacturer’s instructions. Absolute quantification was performed using the

(14) Self-reinoculation with fecal flora changes microbiota density and composition leading to an altered bile-acid profile in the mouse small intestineMicrobiomeDecember 1, 2020Said R. Bogatyrev, Justin C. Rolando, Rustem F. Ismagilovcontained 2.0 μL of the DNA extracts combined with the ddPCR master mix (QX200 ddPCR EvaGreen Supermix, #1864033, Bio-Rad Laboratories), forward and reverse primers (synthesized by Integrated DNA Technologies; Additional

(15) Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR MethodFrontiers in MicrobiologyNovember 19, 2020John Charles Rotondo, Lucia Oton-Gonzalez, Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Mauro Tognon, Fernanda MartiniCatalog number: #1864100. • QX200TM ddPCRTM EvaGreen Supermix (Bio-Rad, Segrate, Italy). Catalog number: #1864035. • DG32TM Automated Droplet Generator Cartridges (BioRad, Segrate, Italy). Catalog number: #1864108. •

(16) Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and DefectsDevelopmental cellJune 8, 2020Harris JM, Wang AY, Boulanger-Weill J, Santoriello C, Foianini S, Lichtman JW, Zon LI, Arlotta P.and mCherry expression in sorted spinal motor neurons using the QX200 Evagreen ddPCR supermix (BioRad 1864033) with a QX200 ddPCR machine (BioRad)(primers in Table S1 (Jacobs, et al., 2018; Rosowski, et al., 2016)).

(17) A Circular RNA from the MDM2 Locus Controls Cell Cycle Progression by Suppressing p53 LevelsMolecular and Cellular BiologyApril 13, 2020Ritu Chaudhary et al.RNA was reversely transcribed, and the PCR using the droplets was generated using EvaGreen Supermix (1864033; Bio-Rad) containing 5 ␮l of cDNA for quantifying the copy numbers of circ-MDM2 or 1 ␮l of 4% (vol/vol)

(18) Accumulation of mitochondrial 7S DNA in idiopathic and LRRK2 associated Parkinson's diseaseEBioMedicineOctober 17, 2019Petar Podlesniy, Margalida PuigrÃ²s, NÃºria Serra, RubÃ©n FernÃ¡ndez-Santiago, Mario Ezquerra, Eduardo Tolosa, Ramon TrullasLaboratories, RRID:SCR_008426). The digital droplet reaction consisted of 1× QX200™ ddPCR™ EvaGreen Supermix (1864033, Bio-Rad, RRID:SCR_008426), an aliquot of target sample (1ul for diploid genome determination and 0,001ul

(19) The Fusion Oncogene FUS-CHOP Drives Sarcomagenesis of High-Grade Spindle Cell Sarcomas in MiceSarcomaJuly 25, 2019Mark Chen, Eric S. Xu, Nathan H. Leisenring, Diana M. Cardona, Lixia Luo, Yan Ma, Andrea Ventura, David G. Kirsch

(20) MicroRNA-Based Prophylaxis in a Mouse Model of Cirrhosis and Liver CancerMolecular therapy. Nucleic acidsMarch 1, 2019Callegari E, Domenicali M, Shankaraiah RC, D'Abundo L, Guerriero P, Giannone F, Baldassarre M, Bassi C, Elamin BK, Zagatti B, et al.for amplification in a 20-μL reaction volume containing ddPCR EvaGreen Supermix and specific primers (1864033; Bio-Rad, Hercules, CA, USA). Droplet generation, cycling conditions, and the enumeration of positive

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